Сенсор бета-лактоглобуліну на основі ІСПТ з підзатворним діелектриком СеО2

Oksana Yuriivna Kutova; Vladimir Ivanovych Tymofieiev; Mykhailo H. Dusheiko; Guillaume Brotons

doi:10.20535/2523-4455.2019.24.4.187940

Vol. 24 · Issue 4 · 2019 Aug 30, 2019 Microsystems and Physical Electronics

Beta-Lactoglobulin Sensor Based on ISFET with CеO2 Gate Dielectric

Oksana Yuriivna Kutova Corresponding Національний технічний університет України "Київський політехнічний інститут імені Ігоря Сікорського" Ukraine

Vladimir Ivanovych Tymofieiev Національний технічний університет України "Київський політехнічний інститут імені Ігоря Сікорського" Ukraine

Mykhailo H. Dusheiko Національний технічний університет України "Київський політехнічний інститут імені Ігоря Сікорського" Ukraine

Guillaume Brotons Université du Maine Institut des Molécules et des Matériaux du Mans France

Pages13-17 PublishedAug 30, 2019

PDF (Ukrainian)

VOL 24 · ISS 4 · 2019 View issue

Abstract

In this article sensor with improved measurement technique for the detection of milk allergens was developed. β-lactoglobulin (BLG) protein was used as the biomarker for cow milk detection. The structure of the sensor with nanocrystalline cerium oxide is proposed with an advanced measurement method, which consists of using a micro-flow system to provide a constant volume of the substance to be tested for the dynamic analysis of the beta-lactoglobulin protein, the major milk allergen. The influence of illumination on the experiment and the degree of cleaning of the sensor surface between each measurement are analyzed. Because the existing ELISA method is too long (1.5 hours), and the proposed method allows to identify the presence of beta-lactoglobulin in a few minutes. U gs = µA

Keywords

#biosensor #β-Lactoglobulin (BLG) #Ion-Sensitive Field-Effect Transistor (ISFET) #Selectivity

References

A. Goossens, "Allergy and hypoallergenic products" Handbook of Cosmetic Science and Technology. 3rd edition. chapter 53. Informa Healthcare pp. 553–562, 2009. DOI: 10.1155/2011/467071.
P. Restani, A. Gaiaschi, A. Plebani, B. Beretta, G. Cavagni, A. Fiocchi, C. Poiesi, T. Velonà, A. G. Ugazio, C. L. Galli, "Cross-reactivity between milk proteins from different animal species" Clinical Experimental Allergy, vol. 29, no. 7, pp. 997–1004, 1999, DOI: 10.1046/j.1365-2222.1999.00563.x.
A. Nowak-Wegrzyn, K. A. Bloom, S. H. Sicherer, W. G. Shreffler, S. Noone, N. Wanich, H. A. Sampson, Tolerance to extensively heated milk in children with cow's milk allergy" Journal of Allergy and Clinical Immunology, vol. 122, no. 2, pp. 342-347, DOI: 10.1016/j.jaci.2008.05.043.
J. Andersson, B. Mattiasson, "Simulated moving bed technology with a simplified approach for protein purification: separation of lactoperoxidase and lactoferrin from whey protein concentrate" Journal of Chromatography A. vol. 1107, no. 1–2, pp. 88-95, 2006 DOI: 10.1016/j.chroma.2005.12.018.
V. Bonfatti, L. Grigoletto, A. Cecchinato, L. Gallo, P. Carnier, "Validation of a new reversed-phase high-performance liquid chromatography method for separation and quantification of bovine milk protein genetic variants" Journal of Chromatography A. 2008. no. 1195, pp. 101-106, DOI: 10.1016/j.chroma.2008.04.075.
E. A. Zvereva, N. I. Smirnova, A. V. Zherdev, B. B. Dzantiev, E. A. Yurova, E. Y. Denisovich, N. A. Zhizhin, V. D. Kharitonov, E. Yu. Agarkova, S. G. Botina, N. V. Ponomareva, E. A. Melnikova, "Development of methods for determination of beta-lactoglobulin in milk and dairy products by immuno- energy analysis", Rational nutrition, nutritional supplements and biostimulants, no. 1, pp. 23-24, 2014
F. Stumr, D. Gabrovska, J. Rysova, P. Hanak, J. Plicka, K. Tomkova, P. Cuhra, M. Kubik, S.Barsova, L. Karsulinovi, H. Bulawova, J. Brychta, " Enzyme-linked immunosorbent assay kit for beta-lactoglobulin determination: interlaboratory study", Journal of AOAC International vol. 92, no. 5, pp. 1519-1525, 2009 DOI: 10.1080/09540100802520755
A. N. Shmyreva, A. V. Borisov, N. V. Maksimchuk, "Electronic Sensors Built on Nanostructured Cerium Oxide Films", Nanotech in Russia no. 5, pp. 382-389, 2010, DOI: 10.1134/S1995078010050137
O. Kutova, M. Dusheiko, T. Obukhova, N. Maksimchuk, T. Borodinova, V. Tymofeev, "H2O2 sensor based on MOSFET with active layer in substrate area", Sensor Electronics and Microsystem Technologies vol. 14, no. 4, pp. 5-12, 2017, DOI: 10.18524/1815-7459.2017.4.116007
O. Kutova, M. Dusheiko, N. I. Klyui, V. A. Skryshevsky, "C-reactive protein detection based on ISFET structure with gate dielectric SiO2 - CeO2", Microelectronic Engineering, vol. 215, no. 15, 110993, 2019 DOI: 10.1016/j.mee.2019.110993.

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[1] A. Goossens, "Allergy and hypoallergenic products" Handbook of Cosmetic Science and Technology. 3rd edition. chapter 53. Informa Healthcare pp. 553–562, 2009. DOI: 10.1155/2011/467071.

[2] P. Restani, A. Gaiaschi, A. Plebani, B. Beretta, G. Cavagni, A. Fiocchi, C. Poiesi, T. Velonà, A. G. Ugazio, C. L. Galli, "Cross-reactivity between milk proteins from different animal species" Clinical Experimental Allergy, vol. 29, no. 7, pp. 997–1004, 1999, DOI: 10.1046/j.1365-2222.1999.00563.x.

[3] A. Nowak-Wegrzyn, K. A. Bloom, S. H. Sicherer, W. G. Shreffler, S. Noone, N. Wanich, H. A. Sampson, Tolerance to extensively heated milk in children with cow's milk allergy" Journal of Allergy and Clinical Immunology, vol. 122, no. 2, pp. 342-347, DOI: 10.1016/j.jaci.2008.05.043.

[4] J. Andersson, B. Mattiasson, "Simulated moving bed technology with a simplified approach for protein purification: separation of lactoperoxidase and lactoferrin from whey protein concentrate" Journal of Chromatography A. vol. 1107, no. 1–2, pp. 88-95, 2006 DOI: 10.1016/j.chroma.2005.12.018.

[5] V. Bonfatti, L. Grigoletto, A. Cecchinato, L. Gallo, P. Carnier, "Validation of a new reversed-phase high-performance liquid chromatography method for separation and quantification of bovine milk protein genetic variants" Journal of Chromatography A. 2008. no. 1195, pp. 101-106, DOI: 10.1016/j.chroma.2008.04.075.

[6] E. A. Zvereva, N. I. Smirnova, A. V. Zherdev, B. B. Dzantiev, E. A. Yurova, E. Y. Denisovich, N. A. Zhizhin, V. D. Kharitonov, E. Yu. Agarkova, S. G. Botina, N. V. Ponomareva, E. A. Melnikova, "Development of methods for determination of beta-lactoglobulin in milk and dairy products by immuno- energy analysis", Rational nutrition, nutritional supplements and biostimulants, no. 1, pp. 23-24, 2014

[7] F. Stumr, D. Gabrovska, J. Rysova, P. Hanak, J. Plicka, K. Tomkova, P. Cuhra, M. Kubik, S.Barsova, L. Karsulinovi, H. Bulawova, J. Brychta, " Enzyme-linked immunosorbent assay kit for beta-lactoglobulin determination: interlaboratory study", Journal of AOAC International vol. 92, no. 5, pp. 1519-1525, 2009 DOI: 10.1080/09540100802520755

[8] A. N. Shmyreva, A. V. Borisov, N. V. Maksimchuk, "Electronic Sensors Built on Nanostructured Cerium Oxide Films", Nanotech in Russia no. 5, pp. 382-389, 2010, DOI: 10.1134/S1995078010050137

[9] O. Kutova, M. Dusheiko, T. Obukhova, N. Maksimchuk, T. Borodinova, V. Tymofeev, "H2O2 sensor based on MOSFET with active layer in substrate area", Sensor Electronics and Microsystem Technologies vol. 14, no. 4, pp. 5-12, 2017, DOI: 10.18524/1815-7459.2017.4.116007

[10] O. Kutova, M. Dusheiko, N. I. Klyui, V. A. Skryshevsky, "C-reactive protein detection based on ISFET structure with gate dielectric SiO2 - CeO2", Microelectronic Engineering, vol. 215, no. 15, 110993, 2019 DOI: 10.1016/j.mee.2019.110993.

Beta-Lactoglobulin Sensor Based on ISFET with CеO2 Gate Dielectric

Abstract

Keywords

References

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