Сенсор бета-лактоглобуліну на основі ІСПТ з підзатворним діелектриком СеО2

Oksana Yuriivna Kutova; Vladimir Ivanovych Tymofieiev; Mykhailo H. Dusheiko; Guillaume Brotons

doi:10.20535/2523-4455.2019.24.4.187940

Vol. 24 · Issue 4 · 2019 Aug 30, 2019 Microsystems and Physical Electronics

Beta-Lactoglobulin Sensor Based on ISFET with CеO2 Gate Dielectric

Oksana Yuriivna Kutova Corresponding Національний технічний університет України "Київський політехнічний інститут імені Ігоря Сікорського" Ukraine

Vladimir Ivanovych Tymofieiev Національний технічний університет України "Київський політехнічний інститут імені Ігоря Сікорського" Ukraine

Mykhailo H. Dusheiko Національний технічний університет України "Київський політехнічний інститут імені Ігоря Сікорського" Ukraine

Guillaume Brotons Université du Maine Institut des Molécules et des Matériaux du Mans France

Pages13-17 PublishedAug 30, 2019

PDF (Ukrainian)

VOL 24 · ISS 4 · 2019 View issue

Abstract

In this article sensor with improved measurement technique for the detection of milk allergens was developed. β-lactoglobulin (BLG) protein was used as the biomarker for cow milk detection. The structure of the sensor with nanocrystalline cerium oxide is proposed with an advanced measurement method, which consists of using a micro-flow system to provide a constant volume of the substance to be tested for the dynamic analysis of the beta-lactoglobulin protein, the major milk allergen. The influence of illumination on the experiment and the degree of cleaning of the sensor surface between each measurement are analyzed. Because the existing ELISA method is too long (1.5 hours), and the proposed method allows to identify the presence of beta-lactoglobulin in a few minutes. U gs = µA

Keywords

#biosensor #β-Lactoglobulin (BLG) #Ion-Sensitive Field-Effect Transistor (ISFET) #Selectivity

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